Josie and the Pussycats are an all-female rock band.
- Josie McCoy - lead singer and guitarist
- Melody Valentine - drummer
- Valerie Brown - backup singer and tambourine player
- Alexander Cabot III - manager
- Alexandra Cabot - sister to Alexander, wanna-be musical star
- Alan M. Mayberry - roadie, Josie's love interest
- Sebastian the Cat - Alexandra's cat