Rose Wilson is one of the three secondary villains in animated television horror film Scooby-Doo: The Watcher in the Barn. She is voiced by Pamela Adlon, who is well known for voicing Andy Johnson in Squirrel Boy, Buford's mother in Phineas and Ferb, Mrs. Levin in Ben 10 series, and Ashley Spinelli in Recess series.
Little is known of Rose Wilson before her first encountered with Fauntleroy Donaldson, but it is known that she was the leader of a group of bikers, and two of her members were Black Knight (Jennifer Hale) and Crayon Boy (Richard Steven Horvitz).
Rose, along with Black Knight and Crayon Boy, stops at a small convenience store in Ponyville. Rose and Black Knight held Kuki Sanban (Lauren Tom) hostage, while Crayon Boy was making trouble for Hoagie Gilligan (Benjamin Diskin). When he has Kuki's thrown green wallet, he wants Hoagie to ask him politely for the wallet back with a word "sir", so Hoagie does. Rose and Black Knight let Kuki go. While she and Hoagie get ready to leave, Rose was outside in front of the store, drinking a can of Sprite soda as she sees Kuki and Hoagie inside the car. When Kuki starts the car, Rose begins smashing the windshield of a car that Kuki was driving. However, Kuki runs over their motorcycles as revenge for troubling her and Hoagie, causing Rose to fume and shout in anger while Hoagie praises Kuki as they drive away.
The bikers stops at the Sweet Apple Acres. Rose goes to a barn across from Applejack's house and discovers Black Knight dead. When she sees Fauntleroy jumping down, she grabs a meat cleaver. Fauntleroy continues his scope out of the barn, only to have Rose appear and start attacking him with a meat cleaver. Fauntleroy counterattacks by hitting Rose with a baseball bat. While Fauntleroy is trying to kill Applejack (Ashleigh Ball), she runs into the barn. She is suddenly met by Rose, who survived the earlier fight. Enraged, Fauntleroy turns his attention to Rose by using a meat cleaver to chops off her left hand. Applejack continues running in fear as Rose screams in horror after Fauntleroy chops off her left hand and hacked her to death multiple times with a meat cleaver. Rose's one-handed corpse was found by the authorities and taken away by the paramedics.