Sometime before the Arkham Asylum events, Batman and his first Robin, Dick Grayson suddenly heard of an apocalypse happening, and they team up with 4 survivors and a dog to find the problem.
- Batman (Roger Craig Smith)
- Scooby-Doo (Kevin Michael Richardson)
- Dick Grayson/Robin (Will Friedle)
- Fred Jones (Freddie Prinze Jr.)
- Daphne Blake (Sarah Michelle Gellar)
- Velma Dinkley (Katie Mucci)
- Shaggy Rogers (Matthew Lillard)
- Commissioner Gordon (J.K. Simmons)
- Scrappy-Doo (Dee Bradley Baker) - in Gotham Bay
- Joker (Troy Baker) - ???
- Penguin (Nolan North) - ???
- Two-Face (John DiMaggio)
- The gang are modeled after their Scooby Apocalypse comic characters to fit in tune with Arkham game series.